Figure 2: Localization of DBN-1 in live C. elegans during muscle contraction.

(a) Schematic representation of the sarcomere lattice in a C. elegans body wall muscle cell. The sarcomeres are oriented at an angle of 5.9° to the muscle long axis. (b) Time series showing dynamics of GFP-DBN-1 distribution in contracting body wall muscle of C. elegans. (c) Schematic localization of DBN-1 in the sarcomere in the relaxed and contracted states. (d) Spatial frequency profiles of GFP-DBN-1 patterns in the relaxed (black) and contracted (red) muscle shown in b. Spatial periods corresponding to the FFT peaks are indicated. (e) Localization of GFP-DBN-1 in body wall muscle after treatment with 10 mM muscimol. (f) Localization of GFP-DBN-1 in body wall muscle after treatment with 5 mM tetramisole. Scale bar, 10 μm.