Figure 5: Direct visualization of actin filaments bundles generated in the presence of DBN-1.

(a) Actin filaments, labelled with rhodamine phalloidin, observed by fluorescence microscopy. (b) Actin filaments in the presence of GST. (c) Bundles of actin filaments formed in the presence of GST-DBN-1 (d) AFM image of a single actin/DBN-1 bundle. (e) High-magnification image of a region of the bundle shown in d. Single molecules of GST-DBN-1 decorated the sides of actin filaments. (f) AFM image of an actin filament in the absence of DBN-1. (g,h) Height profiles along the lines marked in e and f.