Figure 2: The function of Bcl2-L-13 in mitochondrial dynamics. | Nature Communications

Figure 2: The function of Bcl2-L-13 in mitochondrial dynamics.

From: Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation

Figure 2

(a,b) Expression level of Bcl2-L-13 mutants. Immunoblot of whole-cell lysates from HEK293A cells expressing various HA-tagged Bcl2-L-13 constructs 48 h after transfection (a). HEK293A cells were transfected with Bcl2-L-13-specific siRNA (siBcl2-L-13) or with a non-targeting control siRNA (siCTRL), and analysed by immunoblotting with anti-Bcl2-L-13 antibody (b). (c) Mitochondrial morphology in HEK293A cells transfected with empty vector or HA-Bcl2-L-13 was analysed using anti-ATP synthase and anti-HA antibodies. Boxed areas for ATP synthase staining are shown at higher magnification (high) in the right panels. Scale bar, 10 μm. (d) Electron micrographs of mitochondria in HEK293A cells transfected with empty vector or HA-Bcl2-L-13. Images at higher magnification are shown in the right panels. Scale bar, 1 μm in the left panels and 200 nm in the right panels. (e) Representative z-stack confocal images of mitochondrial morphologies in HEK293A cells transfected with siCTRL or siBcl2-L-13 using anti-ATP synthase antibody. Scale bar, 10 μm. (f) Electron micrographs of mitochondria in HEK293A cells transfected with siCTRL or siBcl2-L-13. Images at higher magnification are shown in the right panels. Scale bar, 5 μm in the left panels and 2 μm in the right panels. (g) HEK293A cells transfected with GFP-Bcl2-L-13 were strained with TMRE. Scale bar, 10 μm.

Back to article page