Figure 8: 14-3-3σ is involved in metabolic regulation under hypoxic condition.
From: The cell cycle regulator 14-3-3σ opposes and reverses cancer metabolic reprogramming
(a,b) Induction of Flag-14-3-3σ expression in HCT116 14-3-3σ−/− and MDA-MB-231 cells decreases ECAR in hypoxia. HCT116 14-3-3σ−/− TetR 14-3-3σ cells and MDA-MB-231 TetR 14-3-3σ cells were grown in a hypoxic chamber for 48 h. Flag-14-3-3σ expression was induced for 48 h by adding doxycycline to the culture medium. Noninduced cells were used as a control. ECARs were measured using a Seahorse Extracellular Flux analyzer XF24 (Seahorse Biosciences). n=3; Average±s.d. Student’s t-test, *P<0.05. (c,d) Loss or knockdown of 14-3-3σ results in an increase in glycolytic gene mRNA expression in hypoxia. HCT116 WT (14-3-3σ+/+), HCT116 14-3-3σ−/−, HCT116 Luciferase shRNA, HCT116 14-3-3σ shRNA #419 and HCT116 14-3-3σ shRNA #483 cells were grown in a hypoxic chamber for 48 h. Total mRNA of these cells were extracted and converted to cDNA for real-time PCR reactions with specific primers as indicated above. n=4; average±95% CI; ANOVA,*P<0.05.
