Figure 4: Nanofibrillation of the halogenated pentapeptides.
From: Supramolecular amplification of amyloid self-assembly by iodination
AFM image close-up insets of (a) peptide DF(I)NKF(I), (b) peptide DF(I)NKF, and (c) peptide DF(Br)NKF(Br) evaporated on mica substrates after 9 days incubation in aqueous solutions (scale bar, 1 μm). (d–f) Height profiles of fibrils from a, b and c (cross-section lines highlighted in white). (g) Cross-sectional analysis on the top of a fibril segment in a showing a helicoidal profile along the longitudinal direction of the fibrils (highlighted in black). (h) Cryo-TEM images of the dried hydrogel of DF(I)NKF, showing a network of long fibrils (scale bar, 200 nm). (i) In situ electron tomography of half-pitch helicoidal peptide DF(I)NKF(I) vitrified from aqueous solution. The twisted fibril morphology is highlighted by arrows. Electron tomography reconstructions were collected upon tilting of samples up to ±69° (scale bar, 25 nm).
