Figure 5: Mixed hydrogels formed by co-assembly of DFNKF and DF(I)NKF(I).
From: Supramolecular amplification of amyloid self-assembly by iodination
(a) Photograph of vials after 48 h containing (I) 15 mM DFNKF, which did not form hydrogel; (II) 1.5 mM DF(I)NKF(I), which did not form hydrogel and some precipitation was observed; (III) 15 mM DFNKF:0.15 mM DF(I)NKF(I), which did not form hydrogel; (IV) 15 mM DFNKF:0.75 mM DF(I)NKF(I), which formed hydrogel within 48 h; (V) 15 mM DFNKF:1.5 mM DF(I)NKF(I), which formed hydrogel within 48 h. (b) Rheological characterization of mixed hydrogels by frequency sweep studies whereby the storage concentration of 15 mM after 48 h were utilized for the rheological measurements. (c) AFM of a 15 mM solution of wild-type peptide DFNKF showed small fibrils and aggregates unable to network sufficiently to form a hydrogel. (d) AFM of a sample of the mixed hydrogel containing 15 mM DFNKF and 1.5 mM DF(I)NKF(I) showed larger and more entangled fibrils (scale bar, 400 nm).
