Figure 4: TTP downregulation is essential for neuronal development. | Nature Communications

Figure 4: TTP downregulation is essential for neuronal development.

From: A post-transcriptional mechanism pacing expression of neural genes with precursor cell differentiation status

Figure 4

(a) Dox-inducible TRE-TTP transgene used to express HA-tagged TTP protein in P19 cells. (b) RT–qPCR analyses of EB/RA-differentiated P19-TTP cells showing that transgenic TTP protein induced by Dox (Dox+) diminishes expression of TTP target genes compared with the corresponding Dox− samples. Notably, Dox has no effect on TTP targets in control P19 cells. Data are averaged from three amplifications±s.d. and compared by t-test. (c) Immunoblot analyses of EB/RA-differentiated P19-TTP cells showing that Dox-induced (Dox+) TTP production leads to reduced expression of TubβIII and HuB proteins as compared with the Dox− control. Tubβ is used as a lane loading control. (d) Immunofluorescence analyses of P19-TTP cells with and without Dox. (e) Quantification of the data in d showing that the TTP-expressing (Dox+) culture contains dramatically fewer TubβIII-positive cells than the Dox− control. Data are averaged from 15 randomly selected fields±s.d. and compared by t-test.

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