Figure 5: TTP knockdown in neuroblastoma cells is sufficient for upregulation of ARE-containing neuronal markers. | Nature Communications

Figure 5: TTP knockdown in neuroblastoma cells is sufficient for upregulation of ARE-containing neuronal markers.

From: A post-transcriptional mechanism pacing expression of neural genes with precursor cell differentiation status

Figure 5

(a) RT–qPCR analyses showing significant upregulation of TTP targets after knocking down endogenous TTP (siTTP) in Neuro2a cells. Data are averaged from three experiments±s.d. and compared by t-test. (b) TTP knockdown in Neuro2a cells also stimulates expression of TubβIII and HuB proteins. Tubβ is used as a lane loading control. (c) Immunofluorescence analysis demonstrating increased incidence of TubβIII-positive cells in the siTTP-treated sample compared with siControl. (d) Quantitation of the data in c. Data are averaged from 15 randomly selected fields±s.d. and compared by t-test.

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