Figure 5: Effect of intestinal segment length on scoring variability and sampling strategies for functional assays. | Nature Communications

Figure 5: Effect of intestinal segment length on scoring variability and sampling strategies for functional assays.

From: Stereomicroscopic 3D-pattern profiling of murine and human intestinal inflammation reveals unique structural phenotypes

Figure 5

(a) Effect of length and location of the examined segment on the SM percentage of abnormal mucosa in the small intestine tested from three consultation mouse cases. Length for group was 7–9 cm; ideal is 10 cm. Zero and max values were used in calculation for cm10. Note standard deviation (s.d.) and coefficient of variations (CV=s.d./mean). (b) Contextualization of distribution of the estimated sampling errors for mice in a with respect to their actual experimental (population) group from which animals were randomly chosen. Notice that distribution variability (sampling errors) may prevent the experimenter to statistically detect small treatment effects if sampling is not systematic and if animals had mild/moderate disease (case 2 versus case 7). Boxplots display interquartile ranges, median, min and max values. (c) Graphical illustration of various stereomicroscopic sampling strategy types and suggested best-observed uses. The size of microdissected samples and their functional reproducibility using mRNA qPCR with reverse transcription (qRT–PCR) gene expression data are presented in Supplementary Fig. 11.

Back to article page