Figure 2: Low level of CSR-like recombination in KIKS mice.
From: Efficient AID targeting of switch regions is not sufficient for optimal class switch recombination
(a) Representative flow cytometry analyses of CSR in spleen B cells (B220+) from SRBC-immunized mice. Percentages of B cells expressing IgG1 or murine κ-LC are indicated. (b) Comparison of IgH CSR efficiency in WT, KIKS AID+/+, KIKS AID+/− and KIKS AID−/− mice on SRBC immunization. Percentages of switched B cells in spleen are shown (mean±s.e.m.) (c) Comparison of KIKS CSR efficiency in KIKS AID+/+, KIKS AID+/− and KIKS AID−/− mice on SRBC immunization (days 8 and 10 after boost). Percentages of mκ-LC switched B cells in spleen and PP are shown (mean±s.e.m.). For b,c, n=6 mice of each strain except for KIKS AID−/−, n=4 mice in at least two independent experiments. (d) ELISA quantification of serum mκ-LC (black) as compared with IgG1 (light grey) and IgG3 (dark grey) in non-immunized (NI) and SRBC-immunized mice. Superimposed means (μg ml−1) are shown (n=4–6 mice per group in two independent experiments).(e) Representative flow cytometry analyses of human and mouse κ-LC expression among B220+ cells in PP of KIKS AID+/+, KIKS AID+/− and KIKS AID−/− mice. Percentage of mκ-LC+ on total B cells (B220+) (upper panel) and on GC B cells (B220+ GL7+) is represented (bottom panel).
