Figure 3: Analysis of KIKS CSR-like junctions.
From: Efficient AID targeting of switch regions is not sufficient for optimal class switch recombination
(a) Location of DNA breaks (vertical lines) in Sμ or intron Jκ5 (Int Jκ5) (top) and Sγ3 (bottom) implicated in each sequenced junction. (b) Mutation rates (mutation per kb) in joined Sμ (top) and Sγ3 (bottom) are indicated above the pie charts. Segment sizes in the pie charts are proportional to the number of sequences carrying the number of mutations indicated in the charts. The number of independent sequences analysed is indicated in the centre of each chart. (c) Analysis of microhomology at Sμ/Sγ3 junctions in KIKS GC B cells. Histograms represent the percentage of Sμ/Sγ3 junction sequences with indicated nucleotide overlap. Mean length of overlap in base pairs (OL) and number of sequences analysed (n) are indicated. * Sequences with small insertions (one to three nucleotides) at the junction were scored as zero microhomology and represent 15% of total junctions. For b,c, results were obtained from four independent experiments in which PP of three mice were pooled.
