Figure 4: KIKS locus is accessible to AID.
From: Efficient AID targeting of switch regions is not sufficient for optimal class switch recombination
(a) Analysis of mutations in KIKS Sμ region according to VJ rearrangement. Left panel: the two primers used for amplification (short arrows) allow amplification of KIKS alleles that are rearranged to all the Jκ segments but analyses were carried out on Jκ5 (top) or Jκ1 (bottom) rearrangements. For both Jκ5 and Jκ1 rearrangements, Jκ5 intron and 5′ part of Sμ region were sequenced. Right panel: pie charts analyses of Jκ5 intron and 5′ Sμ mutations from sorted GC (B220+ PNAhigh Fas+) PP B cells from KIKS mice. Segment sizes of the pie charts are proportional to the number of sequences bearing the indicated numbers of mutations. Numbers of analysed sequences (centre of pie charts) and number of mutations per kb (mut per kb) are indicated (below pie charts). (b) Analysis of mutations in KIKS 5′ Sγ3 region from the same cells as in a. Sequenced region, amplified with indicated primers (shorts arrows), is represented. Pie chart features are the same than in a. (c) Analysis of mutations in IgH Sμ region from the same cells as in a. Sequenced region, amplified with indicated primers (shorts arrows) is represented. Pie chart features are the same than in a.
