Table 3 Dissociation constants (KD) for interactions between RING1B–PCGF complexes with either UbcH5c(S22R/C85K)∼Ub or UbcHc(S22R/C85K).

From: BMI1–RING1B is an autoinhibited RING E3 ubiquitin ligase

E3

KD (μM)

 

E2

E2∼Ub

RING1B–PCGF1

1.2±0.1

0.9±0.2

RING1B–PCGF2

7±3

3±1

RING1B–PCGF3

7±2

5±2

RING1B–PCGF4

5.1±0.7

4±1

RING1B–PCGF5

6.1±0.4

2.9±0.5

RING1B–PCGF6

3.1±0.3

2.8±0.3

  1. Values shown are derived from three independent experiments and are shown as averages±s.e.m. Representative binding curves are shown in Supplementary Fig. 3. Boundaries of the RING1B and PCGF constructs are shown in Fig. 1a.
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