Figure 6: Drought stress and ABA cause significant increase of free N termini.
From: Downregulation of N-terminal acetylation triggers ABA-mediated drought responses in Arabidopsis
(a) Correlation between free N termini of soluble proteins and the RWC determined in leaves from 6-week-old wild-type plants after application of drought (N=3–5). (b) Quantification of free N termini in soluble proteins extracted from leaf discs of 6-week-old wild-type plants treated with 50 μM ABA for indicated time (N=4). (c) NAA10 and NAA15 transcript steady-state level in the wild-type leaf discs after application of 50 μM ABA for 6 h. (d) Immunological detection of NatA subunits and two cytosolic control proteins (OAS-TL A, GR2) in wild-type leaf discs after application of 50 μM ABA for 6 h. Coomassie-stained RuBisC/O large subunit (LSU) served as loading control. (e) Quantification of NAA10 and NAA15 signal shown in d after normalization to loading control. The amount of NAA10 or NAA15 in wild-type leaf discs treated with water was set to 100% (N=4). Data are represented as mean±s.e. Asterisks indicate significant differences to wild type. (P<0.05, Student’s t-test).
