Figure 3: Gravitropic stimulation regulates the circadian clock in the root bend.
From: The circadian clock rephases during lateral root organ initiation in Arabidopsis thaliana
(a) Luminescence levels of TOC1:LUC plants were continuously monitored. TOC1:LUC seedlings were grown vertically and imaged after the seeds were placed on plates. (b,c) Luminescence of control seedlings without gravitropic stimulus was monitored in: root tip and upper root (b), stem and leaf (c). (d,e) In contrast to b,c after 4 days a 90° gravitropic stimulus, as indicated by the grey section, was applied for 12 h. After that period, the plate was rotated back to original start position. (b–e) Error bars indicate s.e.; N=2. (f) From seedlings that have been exposed to a gravitropic stimulus at different ages, various tissues (root bend (g), cotyledons (h), upper root (i) and root meristem (j); indicated by circles) were dissected at the same time/age of the seedling (shown in f). (g–j) RNA was prepared and RT–qPCR was used to measure the levels of CCA1 mRNA. Only the root bends show oscillation of CCA1 expression. Error bars indicate s.d.; N=4.
