Figure 1: Phylogenetic analysis of the P4-ATPase protein family of plant, fungal and animal sequences demonstrates the presence of classes, several of which are kingdom specific.
From: A phospholipid uptake system in the model plant Arabidopsis thaliana
The tree with the highest log likelihood (−92499.4933) inferred from a maximum likelihood analysis as described in the Methods section is shown. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Numbers at the nodes reflect the percentage of 500 replicate bootstrap trees, which resolve the clade at the endpoints of that branch. P4-ATPases are phylogenetically split into seven Classes (numbered 1–7) among which Class 1 is further divided into two subclasses, a and b. ALA10 belongs to Class 7, which is specific to plants. Database accession numbers are given in the Methods section. Plantae: ALA, P4-ATPases from Arabidopsis thaliana; Ambtr, Amborella trichopoda; Batpr, Bathycoccus prasinos; Chlva, Chlorella variabili; Cr, Chlamydomonas reinhardtii; Micpu, Micromonas pusilla; Pp, Physcomitrella patens; Os, Oryza sativa; Ot, Ostreococcus tauri; Sm, Selaginella moellendorffii; and Volca, Volvox carteri; Fungi: Drs2p, Dnf1p, Dnf2p, Dnf3p, and Neo1p, P4-ATPases from Saccharomyces cerevisiae; Agabi, Agaricus bisporus; Aspi, Aspergillus nidulans; Copci, Coprinopsis cinerea; Fo, Fusarium oxysporum; Neucr, Neurospora crassa; Pucgr, Puccinia graminis; Schpo, Schizosaccharomyces pombe; and Walse, Wallemia sebi; Animalia: ATP8-11, P4-ATPases from Homo sapiens; Galga, Gallus gallus; Danre, Danio rerio; Dappu, Daphnia pulex; Drome, Drosophila melanogaster; tat1-5, P4-ATPases from Caenorhabditis elegans; Hydvu, Hydra vulgaris; Ampqu, Amphimedon queenslandica; Triad, Trichoplax adhaerens; and SERCA2a, P2-type (non-P4) ATPase from Oryctolagus cuniculus (P05023). Intracellular localization of P4-ATPases from A. thaliana, S. cerevisiae and H. sapiens12 is indicated as follows: triangles, plasma membrane; asterisks, trans-Golgi apparatus; crosses, endomembranes (endosomes, early endosomes, trans-Golgi apparatus). Blue, fungi isoforms; green, plantae isoforms; and red, animalia isoforms. Scale bar, 0.2 amino-acid substitution per site.
