Figure 2: ALA10 localizes to the plasma membrane when expressed in tobacco.
From: A phospholipid uptake system in the model plant Arabidopsis thaliana
(a) GFP-ALA10 was transiently expressed in tobacco epidermal cells, in concert with a YFP fusion of the HDEL ER retention signal. The GFP and YFP signals overlapped. (b) GFP-ALA10 was transiently expressed in tobacco epidermal cells, in the presence of ALIS1-YFP. The proteins co-localized at a membrane structure that follows the contour of the cell. In plasmolysed cells, bidirectional arrows mark the position of the plasma membrane with respect to the cell wall at places where the membrane has contracted due to cell shrinkage. Connections between the plasma membrane and the cell wall (Hechtian strands) are marked by directional arrows. (c) GFP-ALA10 was transiently expressed in tobacco epidermal cells, in the presence of untagged ALIS1. To avoid artefacts due to overexpression, only cells with low GFP signal were visualized. Indicative of plasma membrane localization, GFP-ALA10 localizes to a membrane structure that follows the contour of the cell but not the nucleus (arrowhead). Scale bar, 25 μm.
