Figure 5: PirtN14 peptide inhibited P2X3 and alleviated the bladder overactivity of Pirt−/− mice.
From: Pirt reduces bladder overactivity by inhibiting purinergic receptor P2X3
(a) An example of an immunoblot obtained from competition assays. (b) A dose-dependence curve was obtained after fitting a single exponential decay curve to the relative intensities obtained for the immunoblot bands; n=4 independent experiments. (c,d) The HEK293T cells expressing P2X3 were preincubated with 10 μM PirtN14 peptide (PirtN14 group), 10 μM scrambled peptide (scramble group) or buffer (control group) for 1 h. (c) The sample traces of inward currents induced by 10 μM α,β-meATP. (d) The histogram of the current densities of P2X3. *P<0.05, versus control; one-way analysis of variance (ANOVA) and Tukey post hoc means comparisons. n=30. (e,f) The acute dissociated lumbosacral DRG neurons from Pirt KO mice were preincubated with 10 μM PirtN14 peptide (Pirt−/−+PirtN14), 10 μM scrambled peptide (Pirt−/−+scramble) or buffer (Pirt−/−) for 1 h. (e) The sample traces of inward currents induced by 30 μM α,β-meATP for 2 s in DRG neurons with DiI fluorescence. (f) The histogram of the current densities of P2X3. *P<0.05, versus Pirt−/−; one-way ANOVA and Tukey post hoc means comparisons. n=10. (g) The sample traces of cystometry recordings. PirtN14 or scrambled peptides were applied at a dosage of 20 mg kg−1. (h) The histogram of the voiding contraction times in 15 min. **P<0.01, versus Pirt−/−; one-way ANOVA and Tukey post hoc means comparisons. n=7. (i) The histogram of the voided volume. **P<0.01, versus Pirt−/−; one-way ANOVA and Tukey post hoc means comparisons. n=6. Error bars represent s.e.m.
