Figure 3: The intrinsic excitability of layer 2/3 neurons is decreased in the contralateral PL 1 day after CFA injection. | Nature Communications

Figure 3: The intrinsic excitability of layer 2/3 neurons is decreased in the contralateral PL 1 day after CFA injection.

From: Deactivation of excitatory neurons in the prelimbic cortex via Cdk5 promotes pain sensation and anxiety

Figure 3

(a) Current-clamp recordings to identify excitatory neurons in the PL. (b) Representative sEPSC recording traces from the naive rats and the rats 1 day after CFA. Scale bars, 20 pA and 250 ms. (c, d) Cumulative probabilities (***P<0.001, Kolmogorov–Smirnov two sample test) and average sEPSC frequencies and amplitudes from the naive rats (frequency: 2.154±0.1671 Hz, amplitude: 15.67±0.5745, pA, n=21 neurons) and the rats 1 day after CFA (frequency: 4.3324±0.4222 Hz, amplitude: 18.93±0.9648, pA, n=16 neurons, **P<0.01, ***P<0.001, two-tailed t-test). (e) Recording paradigm of the excitatory neurons in the PL. (f) Number of spikes induced by injected currents in the contralateral PL excitatory neurons from the naive rats and the rats 1 day after CFA (n=13, 15 neurons, *P<0.05, **P<0.01, ***P<0.001, two-way ANOVA with Bonferroni post tests). (g) Examples of the AP responses to positive current steps recorded from pyramidal neurons from the naive rats and the rats 1 day after CFA. (h) The current required to drive APs at a frequency of 8.75 Hz from naive rats (200±15.37 pA, n=12 neurons) and the rats 1 day after CFA (280±13.66 pA, n=15 neurons; ***P<0.001, two-tailed t-test). (i) Phase plots of the APs from the naive rats and the rats 1 day after CFA evoked by brief current injections. (j) Plot diagram summary of the AP thresholds from the naive rats (−37.29±1.185 mV, n=12 neurons) and the rats 1 day after CFA (−32.95±0.7555, mV, n=15 neurons; **P<0.01, two-tailed t-test). The data are presented as mean±s.e.m.

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