Figure 2: Telomerase inactivation-induced replicative senescence in single lineages.
From: Two routes to senescence revealed by real-time analysis of telomerase-negative single lineages
(a) Schematic representation of senescence-tracking in the TetO2-TLC1 strain (as in Fig. 1a). (b) Sequential phase contrast and fluorescence images (as in Fig. 1c) of a TetO2-TLC1 cell lineage. Addition of doxycyline renders the lineage telomerase-negative. (c) Display of TetO2-TLC1 lineages (yT528, n=40). Cells were grown in microfluidic chambers for several generations before the addition of doxycycline (30 μg ml−1 in the flowing medium) to inactivate telomerase at generation 0. An ellipsis (…) indicates that the cell was alive at the end of the experiment and X indicates cell death. A grey segment indicates that the cell cycle was not complete at the end of the experiment.
