Figure 6: Pol32 is required for type B lineages.
From: Two routes to senescence revealed by real-time analysis of telomerase-negative single lineages
(a) Analysis of telomerase-negative pol32Δ lineages (yT662, n=27). (b) Analysis of telomerase-positive pol32Δ lineages (yT662, n=23). (c) Telomerase-negative pol32Δ lineages were clustered into type A and type B lineages. (d) Quantification of the proportion of type B lineages in the indicated strains. The ‘TetO2-TLC1 (long) +dox’ strain corresponds to the strain with longer initial telomeres shown in Supplementary Fig. 8 and Supplementary Fig. 7a, lanes 3 and 4, which displayed a higher frequency of type B lineages. (e) Quantification of the frequency of non-terminal arrests in the indicated strains, used as an alternative assessment of the proportion of type B lineages.
