Figure 3: Adiponectin negatively regulates the recruitment of inflammatory cells and the IL-17 production from γδ-T cells in the skin.

Wild-type (WT) and Adiponectin knockout (Adipo KO) mice were treated as described. At day 5, dermal cell suspensions of mouse back skin were stimulated with PMA and ionomycin, and were stained with antibodies specific for IL-17A as well as CD3 and TCRγδ cell surface markers, followed by flow cytometric analysis. Gating stragedy is shown in Supplementary Fig. 3. (a,c,e,f) Flow plots show the CD3-positive (a), TCRγδ-positive (c,f), IL-17A-positive (e) population of the dermal cells from WT and Adipo KO mice. Flow plots were gated on CD3-positive cells (c,e). (b,d,g,h) Graph shows the percentage of CD3-positive (b), TCRγδ-positive (d,h) and IL-17-positive (g) cells. The percentage of IL-17-positive cells among CD3 cells of each group is shown in Supplementary Table 1. Values are presented as mean±s.e. (n=6). Data are representative of three independent experiments, where each group contains 1 to 2 mice. Data are analysed by Welch’s t-test for a,b,g,h. IMQ, imiquimod.