Figure 4: Vγ4 T cells predominantly induce the skin inflammation and are the major IL-17 producers.
From: Adiponectin regulates psoriasiform skin inflammation by suppressing IL-17 production from γδ-T cells

Wild-type (WT) and Adiponectin knockout (Adipo KO) mice were treated as described. At day 5, whole-skin cell suspensions of mouse back skin were stimulated with PMA and ionomycin, and were stained with antibodies specific for IL-17A as well as CD3, TCRγδ, Vγ4 and Vγ5 cell surface markers, followed by flow cytometric analysis. (a) Dermal γδ-T cells after IMQ application and epidermal γδ-T cells before IMQ application were sorted, and mRNAs were extracted to perform reverse transcription–PCR. (b) Plots show two populations of γδ-T cells, composed of TCRγδintermediate IL-17-positive (I) and TCRγδhigh IL-17-negative (II) cells. (c) Cells of (I) and (II) populations were characterized for TCRγδ and Vγ4 or Vγ5 expression. (d) IL-17-positive cells gated on CD3-positive cells were characterized for Vγ4 and Vγ5 expression. Data are representative of two independent experiments, where each group contains two mice.