Figure 1: Hepatic development of P. falciparum in LH TK-NOG mice.
From: Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice
Representative pictures of P. falciparum schizonts at days 5 and 7 post-infection. Sixteen-μm thick frozen and 5-μm thick deparaffinized liver sections were stained by indirect immunofluorescence with antibodies specific to P. falciparum and DAPI to stain host cell and parasite nuclei. (a). Day-5 and day-7 schizonts stained for Plasmodium HSP70, scale bar, 20 μm. (b). Maximal diameter and area of day-5 and day-7 schizonts; n=32 at day 5 and n=27 at day 7, both from 3 different lobes, mean±s.d. ***P<0.0001, Mann–Whitney U-test. (c). Day-7 schizonts stained for EXP-1 and SERP. (d). Schizonts were stained for MSP-1, AMA-1 and RON-4 at day 5 and day 7. Scale bar, 20 μm. (e). Merosome-like structures stained for HSP70 and DAPI observed in day-7 schizonts, left picture, Scale bar, 20 μm; central picture, magnification of left picture showing merozoites nuclei in the merosome-like structure, Scale bar, 10 μm; right picture, Scale bar, 50 μm.
