Figure 3: Hepatic development of P. ovale in LH-TK-NOG mice.
From: Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice
Representative pictures of P. ovale schizonts and hypnozoites at days 8 and 21 post-infection. 50-μm thick frozen (a,b) or 5-μm thick deparaffinized liver sections (d) were stained by indirect immunofluorescence with immune serum specific to Plasmodium HSP70 and DAPI to stain host cell and parasite nuclei. (a). Day-8 schizonts, scale bar, 20 μm. (b). Upper panel: day-8 schizont and—hypnozoite (white arrow), scale bar, 20 μm. Lower panel: higher magnification of the hypnozoite showing the single nucleus within the 5 μm round-shape parasite, scale bar, 5 μm. (c) Maximal diameter and area of day-8 schizonts; n=35, from three different lobes, mean±s.d. (d). Day-21 schizont, scale bar, 20 μm.
