Figure 4: Pax7Lo satellite cells of miR-431 TG mice are more primed for differentiation than the WT controls. | Nature Communications

Figure 4: Pax7Lo satellite cells of miR-431 TG mice are more primed for differentiation than the WT controls.

From: MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

Figure 4

(a) Satellite cells from the Pax7Cre::R26mTmG::miR-431 and Pax7Cre::R26mTmG controls were sorted using FACS, cultured for 48 h in growth medium and immunostained for MyoD (green) and MyoG (red). Blue, 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining. Scale bars, 100 μm. (b) Numbers of the MyoD+ and MyoG+ cells in a. Values are means±s.e.m. of triplicate experiments. *P<0.05, **P<0.01. Two-tailed Student’s t-test was used for all statistical testings. (c) Double staining for Pax7 and MyoD in expanded satellite cells from single fibres isolated from EDL muscle and cultured for 72 h. The data are from five mice for each genotype and more than 200 myofibres were analysed. Scale bar, 50 μm. (d) Numbers of Pax7+/MyoD+, Pax7–/MyoD+ and Pax7+/MyoD– cells in cultured single fibres from miR-431 TG and WT mice in c. Values are means±s.e.m. **P<0.01, ***P < 0.001. Two-tailed Student’s t-test was used for all statistical testings.

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