Figure 5: miR-431 TG mice exhibit accelerated skeletal muscle regeneration.

The H and O lines of the miR-431 TG mice had similar phenotypes. The data presented here are from the analysis of H line mice. Values are mean±s.e.m. or one representative value from five pairs of WT and TG mice. *P<0.05, **P<0.01. NS, not significant. Two-tailed Student’s t-test was used for all statistical testings. (a) Representative haematoxylin and eosin (H&E)-stained sections of TA muscle 7 days after injury induced by CTX injection. Controls were injected with PBS. Scale bar, 50 μm. (b) Cross-sectional area of regenerated myofibres with centralized nuclei, calculated from the H&E-stained sections in a,c. A representative view of MyoD staining on sections of TA muscle 1 day after CTX injury. Green, MyoD; red, DAPI. Scale bar, 50 μm. (d) Number of MyoD⁺ cells in sections of TA muscle 1 day after CTX injury (shown in c). (e) Western blots showing expression levels of MyoD and Pax7 in TA muscle 1 day after CTX injury. GAPDH was the loading control. (f) Representative sections of TA muscle 3 days after CTX injury, double-stained for Pax7 (red) and MyoD (green). Scale bar, 50 μm. (g) Number of Pax7⁺/MyoD⁺ double-positive cells in sections of TA muscle 3 days after CTX injury (shown in f). (h) Western blots showing expression of MyoD and Pax7 in TA muscle 3 days after CTX injury. GAPDH was the loading control. (i,j) Expression of eMHC in TA muscle 3 days (i) and 7 days (j) after injury, detected using RT–qPCR.