Figure 6: miR-431 does not deplete the satellite pool after two rounds of injury.

One representative value or means±s.e.m. from five pairs of WT and TG mice. *P<0.05. Two-tailed Student’s t-test was used for all statistical testings. (a) Pax7 staining on sections of CTX-damaged TA muscles from miR-431 TG and WT mice 31 days after injury (d.p.i.). Scale bar, 50 μm. (b) Number of Pax7-positive cells in the stained sections in a. (c) Western blots showing Pax7 expression in injured TA muscle 31 d.p.i. (d,e) Pax7 and myogenin gene expression in the samples in c were determined using real-time RT–PCR. GAPDH was the internal control. (f) Newly formed regenerated myofibres with centralized nuclei in H&E-stained sections of TA muscle 5 days after the second round of CTX injury. Scale bar, 50 μm. (g) Numbers of regenerated myofibres in sections in f,h. MyoG gene expression in the samples in f, analysed using real-time RT–PCR. GAPDH was the internal control. (i) Number of Pax7-positive cells in sections of TA muscle at 31 days after the second round of CTX-induced injury. Pax7 expression in the same samples was examined using real-time RT–PCR.