Figure 4: Mitochondrial Hst4p localization depends on biotin, and H3K56 acetylation is unaffected in the hst4Δ mutant or by biotin availability. | Nature Communications

Figure 4: Mitochondrial Hst4p localization depends on biotin, and H3K56 acetylation is unaffected in the hst4Δ mutant or by biotin availability.

From: Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p

Figure 4

(a) Biotin and cell-cycle-dependent localization of Hst4–GFP and mitochondrial marker Cox4–RFP in G1, S and G2. Biotin starvation led to a marked accumulation of Hst4–GFP in the mitochondria evidenced by co-localization with Cox4 and 4,6-diamidino-2-phenylindole (DAPI) staining. Scale bar, 2 μm. (b) Hst4–GFP expression dependent on biotin availability shown by western blot analysis on whole-cell extracts. (c) H3K56 acetylation is unaffected by biotin availability in both WT and hst4Δ genetic background, as evidenced by western blot analysis. Control blots for complete biotin depletion was performed. (d) Hst4–GFP expression in mitochondrial fractions on biotin availability. Control blot for mitochondrial fraction using Porin specific antibody. See Supplementary Fig. 3 for additional controls.

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