Figure 5: Anti-GPIbα mAbs induce platelet activation and desialylation in vivo. | Nature Communications

Figure 5: Anti-GPIbα mAbs induce platelet activation and desialylation in vivo.

From: Desialylation is a mechanism of Fc-independent platelet clearance and a therapeutic target in immune thrombocytopenia

Figure 5

(a,b) ITP was induced by intraperitoneal injection of anti-GPIbα mAbs (NIT A, NIT B, NIT E, NIT F, NIT G and NIT H) or sera. At 16 h post injection, platelets isolated from the mice were examined for activation (P-selectin) and desialylation (RCA-1) by flow cytometry; n=3 per mAb. (a) Representative dot plots of isolated washed platelets from anti-GPIbα mAb (NIT E)- and sera-injected mice double-stained for anti-mouse IgG (against anti-GPIbα antibodies) (x axis) and RCA-1/P-selectin (y axis).

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