Figure 8: Sialidase inhibition rescues thrombocytopenia in a murine model of ITP.

(a–c) DANA or PBS was injected intraperitoneally immediately prior to anti-GPIbα or anti-GPIIbIIIa antibody injection (mAb or sera) to induce thrombocyotopenia. (a) Platelets from antibody-injected mice were analysed for desialylation (RCA-1 binding) via flow cytometry. (b) Platelet numbers were enumerated and compared between DANA-treated or mock (PBS)-treated groups. (c) Cumulative platelet counts from all anti-GPIbα and anti-GPIIbIIIa antibody (9D2, M1, PSI C1 and antisera)-injected mice with or without DANA treatment. For individual platelet counts of anti-GPIIbIIIa-injected mice, please see Supplementary Fig. 7. (d) Oseltamivir phosphate was injected intravenously 2 h after anti-GPIbα mAbs injection (equal ratio mixture of individual mAb clones). Platelet enumeration was as described above. Data are shown as mean±s.e.m. platelet counts of individual mAbs. *P<0.05, ***P<0.001 as determined by the Student’s t-test.