Figure 3: Loss of cohibin but not kinesin-14 abrogates perinuclear telomere tethering.
From: Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process
(a) Microscopy approach used to assess the position of a GFP-tagged TEL-XI-L relative to the Nup49-GFP-marked nuclear periphery. The tagged telomere can be localized to one of three concentric nuclear zones of equal area. Scale bar, 1 μm. (b,c) Deletion of Lrs4, but not Cik1, strongly abrogates perinuclear telomere positioning during both S phase (b) and G1 phase (c) of the cell cycle. Percentages are presented for the proportion of cells with the telomere located in different nuclear zones (N=100 per genotype). The χ2 test P values immediately above bars compare telomere positioning relative to a random (dotted line) distribution (*, χ2 test P<0.05). Additional statistical comparisons of cells with different genotypes are as presented. (d) Lrs4 or Cik1 deletion does not affect perinuclear Nup84 localization. Anti-Nup84 immunofluorescence was conducted and the ratio between signal at the nuclear envelope over nucleoplasmic signal was determined; results are presented relative to the wild-type ratio (mean±s.d.; N=10).
