Figure 5: Artificial targeting of a subtelomeric DSB to Nup84 hyperactivates BIR.
From: Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process
(a) Schematic illustrating the insertion position of the DNA zip or control sequence near the subtelomeric (SubTEL) DSB site. (b) Anti-Myc ChIP reveals that the zip, but not control sequence increases interactions between Myc-tagged Nup84 and the uninduced DSB site, but not control TEL-VI-R. Quantification from three independent experiments are presented (mean±s.d.; N=3; *, t-test P≤0.01). (c) Representative anti-Myc ChIP gel is shown and relative fold enrichments for a region near the subtelomeric DSB site or control TEL-VI-R were calculated for cells harbouring zip or control sequences. Results are normalized to ACT1 and presented relative to wild-type controls. (d) DSB assay revealing that zip, but not control sequence, hyperactivates BIR-dependent repair in wild type but not cells lacking Lrs4, Cik1 or Nup84 (mean±s.d., N=5). P values (t-test) comparing control mutants with control wild-type cells are shown immediately above bars. Statistical comparison of zip and control pair are also presented for each of the mutants. Survival analysis was conducted as in Fig. 1c,d.
