Figure 5: GABAergic control of neocortical network activity in vivo.
From: GABA depolarizes immature neurons and inhibits network activity in the neonatal neocortex in vivo
(a,e,i,m) Sample raster plots in the presence of bumetanide (a) diazepam (e) gabazine (i) and L-655,708 (m) from four different mice. Insets (right) show GCaMP3 fluorescence overlaid with binary area plots of Ca2+ clusters as indicated on the left. Scale bars, 200 μm. (b,f,j,n) Distributions of the frequency of Ca2+ transients per ROI. The mean (solid line)±s.e.m. (shaded area). (c,g,k,o) Cluster frequency (mean±s.e.m.) in control versus bumetanide (P>0.2, n=5 mice), control versus diazepam (P>0.1, n=6 mice), control versus gabazine (P<0.001, n=5 mice) and control versus L-655,708 (P>0.4, n=5 mice; paired t-tests). Each pair of symbols represents a single animal. ***P<0.001, n.s., not significant. (d,h,l,p) Distributions of the number of active ROIs per Ca2+ cluster event. Data presented as the mean (solid line)±s.e.m. (shaded area). Insets in d,h,p show boxed region at higher magnification.
