Figure 2: Nanometre super-localization accuracy of a 100 nm gold nanoparticle.
From: Three-dimensional nanometre localization of nanoparticles to enhance super-resolution microscopy
(a) Scatter plot of the nanoparticle lateral x and axial z positions showing 400 measurements at 50 frames per second video rate (20 ms acquisition time per measurement, the numerical computation time is negligible). The x and z position histograms are represented in grey. (b) s.d. of the measured nanoparticle position along x (black line) and z (red dashed line) as a function of the calibrated axial defocus induced by the piezo stage. For each axial stage position, 100 measurements are taken at 50 Hz to estimate the s.d. of the localization. The noise level corresponding to the set-up vibrations over the 2 s integration time is represented by a grey dashed line for the x direction and by an orange dashed dot line for the z direction. (c) Measured axial position as a function of the calibrated piezo stage z displacement.
