Figure 4: Effector binding of covalently modified Rab proteins.

Gel filtration experiments were performed to test for the interaction of the effector protein Mical with Rab1_WT:GppNHp (a) and the covalently locked variants Rab1_E35C–aGppNHp (b) and Rab1_L125C–aGppNHp (c).