Table 1 X-ray data statistics.
Ypt7 Q35C –aGDP | Ypt7 Q35C, K38I, Q68L –aGTP | Ypt7 Q35C –aGppNHp | |
|---|---|---|---|
Data collection* | |||
Space group | P212121 (19) | P21 (4) | P21 (4) |
Cell dimensions | |||
a, b, c (Å) | 49.31, 54.90, 60.47 | 32.97, 53.16, 46.91 | 60.57, 72.23, 82.34 |
α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 106.353, 90.0 | 90.0, 91.485, 90.0 |
Wavelength (Å) | 1.0 | 1.0 | 0.92045 |
Resolution (Å) | 40.65–1.95 | 45.01–1.9 | 48.18–2.35 |
Highest shell | 2.05–1.95 | 2.0–1.9 | 2.45–2.35 |
Rsym (%) | 14.1 (54.3) | 7.0 (36.2) | 5.8 (55.5) |
Rmeas (%) | 14.8 (57.0) | 8.3 (43.3) | 6.2 (59.6) |
I/σ(I) | 12.41 (5.17) | 11.15 (2.85) | 18.28 (3.18) |
Completeness (%) | 100 (100) | 99.5 (99.8) | 99.9 (99.8) |
Redundancy | 12.1 (11.1) | 3.3 (3.2) | 6.9 (7.0) |
Refinement | |||
Resolution | 40.65–1.95 | 45.01–1.9 | 48.18–2.35 |
Highest shell | 2.0–1.95 | 1.95–1.9 | 2.41–2.35 |
No. of reflections | 11,832 | 11,694 | 28,232 |
Rwork (%) | 19.6 (22.9) | 17.5 (23.3) | 19.9 (29.2) |
Rfree (%) | 24.7 (32.9) | 23.2 (34.3) | 26.3 (33.1) |
No. of atoms | |||
Protein | 1,266 | 1,361 | 5,155 |
Ligands | 41 | 44 | 168 |
Water | 60 | 52 | 17 |
B-factors | |||
Protein | 25.8 | 31.4 | 75.6 |
Ligands | 28.7 | 26.7 | 71.5 |
Water | 26.7 | 29.2 | 58.1 |
R.m.s deviations | |||
Bond length (Å) | 0.019 | 0.019 | 0.015 |
Bond angles (°) | 1.859 | 1.910 | 1.672 |
PDB entry code | 4PHF | 4PHG | 4PHH |
