Figure 2: Monocyte-derived origin of gut CD169+ macrophages. | Nature Communications

Figure 2: Monocyte-derived origin of gut CD169+ macrophages.

From: Intestinal CD169+ macrophages initiate mucosal inflammation by secreting CCL8 that recruits inflammatory monocytes

Figure 2

(a) Expression of YFP in the LP myeloid cells from CD169-Cre-YFP reporter mice. LP myeloid cells in the colon were stained for Ly6C, CD64 and Siglec-F or CD103 and fractionated as in Fig. 1a. Numbers indicate frequencies of YFP+ cells among each fraction. Shadows indicate WT control. Representative of two independent experiments. (b) Rosa26-YFP mice (host, left) or CD169-DTR mice (host, right) were parabiotically joined with CD169-Cre-YFP mice (donor). DT was injected into CD169-Cre-YFP and CD169-DTR parabionts (right) 1 week before separation. LP myeloid cells were analysed by flow cytometry for the presence of YFP+ cells 5 weeks after initial cojoinment. Numbers indicate frequencies among 7AAD− LP myeloid cells. Representatives of four pairs are shown.

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