Figure 7: Amelioration of DSS-induced colitis by anti-CCL8-neutralizing antibody.

(a) WT mice were administered with 3% DSS in drinking water for 7 days. One hundred micrograms of anti-CCL8 antibody (clone 17D6, white squares) or isotype IgG (black squares) was injected intravenously into the mice on days 3 and 4. Body weight change relative to the initial value was plotted up to 14 days after the DSS administration. Values are averages and s.e.m. of four to six mice per group. Representative data of four independent experiments are shown. *P<0.05, two-way analysis of variance with multiple comparison. (b) Macroscopic observation of the colon 7 days after the administration of DSS from WT mice injected with isotype IgG (top two colons) or anti-CCL8 antibody (bottom two colons). (c) Average lengths and s.d. of four colons are shown. *P<0.05, Student’s t-test. (d) Haematoxylin and eosin staining of colon section from WT mice that received DSS for 5 days. The mice were treated with isotype IgG (left) or anti-CCL8 antibody (right). Representative images of four mice per group are shown. Scale bar, 200 μm. Original magnification, × 20. (e) IL-17 and IL-22 mRNA expression levels in colon tissue of WT mice on day 7, which were treated with isotype IgG (black bar) or anti-CCL8 antibody (white bar) were determined by qRT–PCR. Expression levels are shown as fold induction relative to the expression level in WT naive colon. Average values and s.e.m. of four colons are shown. P<0.05. NS, not significant, Student’s t-test. Representative data of three independent experiments are shown.