Figure 3: TFBS activation of synthetic core elements creates inducible promoters.
From: The development and characterization of synthetic minimal yeast promoters
(a) Core elements were paired with a minimal GBS. Specifically shown here is UASG4BS4 to develop short, minimal galactose-inducible promoters. Lengths of promoters are illustrated to scale. Scale bar, 100 bp. (b) When linked with certain core elements, the GBS was able to establish short, galactose-inducible promoters with a fully induced strength comparable to that of the GAL1 native promoter. (c) Of the 18 core elements tested in this construct, two did not activate with both UASG4BS3 and UASG4BS4. For demonstrative purposes, one rejected core element is shown here, which does not activate with UASG4BS4. Error bars represent s.d. among biological triplicate.
