Figure 4: Minimal core elements and UAS sequences.
From: The development and characterization of synthetic minimal yeast promoters
TFBS are indicated by arrows with direction of arrow designating the orientation of site. Protein predicted to bind to these sites are labelled as so. (a) The final set of nine core elements established here are distinct from one another spanning a %GC content of 47–73. The quantity, quality and directionality of predicted TFBS, as determined by YEASTRACT, vary greatly. These promoters also display varying dependences to SAGA complex. In a Δspt3 strain, strengths of core elements assembled in three promoter contexts (just core, with UASCIT and with UASG4BS4) were determined. SAGA dependency score of 3 indicates that all three promoters’ strengths were affected in this knockout strain, while a score of 0 indicates that none of the promoters’ strengths were disrupted. High minimum Evalue obtained from BLAST confirm core elements’ uniqueness. (b) Six 10 bp minimal UAS sequences isolated through the library-based sorting and selection.
