Figure 5: Fully synthetic minimal promoters can drive high expression.
From: The development and characterization of synthetic minimal yeast promoters
By linking minimal UAS elements with a core promoter element, synthetic promoters approaching GPD strength can be assembled. (a) Lengths of promoters are illustrated to scale. Scale bar is provided. All synthetic UAS shown (UASF, UASE and UASC) are positioned upstream of the core element using a singular AT-rich neutral 30 bp spacer. Size scale bars are provided. (b) All synthetic UASs can activate core element to levels comparable to the strength of the commonly used promoters CYC1. When assembled in a hybrid fashion, tandem UAS elements can amplify the strength of these core elements with strengths approaching GPD (TDH3) in <20% of the DNA. Error bars represent s.d. among the biological triplicate.
