Figure 3: Targeting negative regulatory nodes in primary macrophages enhances oxidative burst.
From: Functional genomics identifies negative regulatory nodes controlling phagocyte oxidative burst
Primary murine bone marrow-derived macrophages were transduced with shRNA targeting Rbpj (a), Pfkl (b), Rnf145 (c), or controls targeting luciferase or LacZ. Cells were left unprimed or primed with either IFN-γ or IL-4 prior to stimulation with zymosan to induce oxidative burst. Data represent ROS production as relative luminescence units (RLU) measured with luminol substrate. Target gene knockdown was measured by qPCR and normalized to Actb housekeeping gene. Data represent mean±s.d. of biological triplicates.
