Figure 7: HDX studies of open peptide ligand-bound wild-type GCGR versus closed apo H89C/H372C mutant GCGR.
From: Conformational states of the full-length glucagon receptor
Changes to average percent deuterium of extracellular regions observed in HDX studies are shown on full-length models of peptide ligand-bound wild-type GCGR (left) and closed H89C/H372C mutant GCGR (right). The N-terminal region of the ECD (N-term) and TM1 stalk are depicted in dark blue, indicating large decreased exchange in the presence of the des-His1-[Nle9-Ala11-Ala16]-glucagon-NH2 peptide ligand (green), while the smaller decreased exchange of the ECL1 region is coloured cyan. The ECD is coloured magenta, while residues C89 and C372 are coloured yellow. Differences in average percent deuterium values are reported in Supplementary Table 2.
