Figure 4: TaALMT1 currents are activated by external malate at pH 7.5 and are regulated by GABA, muscimol and bicuculline. | Nature Communications

Figure 4: TaALMT1 currents are activated by external malate at pH 7.5 and are regulated by GABA, muscimol and bicuculline.

From: GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters

Figure 4

All results from X. laevis oocytes injected with TaALMT1 cRNA bathed at pH 7.5 and measured using two-electrode voltage-clamp electrophysiology. (a) Representative current traces at −120 mV; M=10 mM malate; Mus=10 μm muscimol; G=100 μm GABA; Bic=100 μm bicuculline from n=5 biological replicates for top and middle traces, n=3 for the bottom trace. Response of water-injected control oocytes are shown in Supplementary Fig. 6. (b,c) Current–voltage relationship of malate-activated current as regulated by muscimol and GABA as indicated applied and recorded 30 s after each solution change. Control-subtracted currents were normalized to the largest mean outward current at +60 mV (n=5 independent oocytes for each treatment). (d) Concentration dependence of GABA- and muscimol-regulated inward current at −140 mV taken from b and c (n=5 for b, and 9 for c). All data are water-control subtracted except in a. All error bars are ±s.e.m. Experiments repeated with oocytes from at least two different frogs.

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