Figure 6: GABA regulation of transport activity is a common ALMT property as is the presence of a putative GABA-binding motif. | Nature Communications

Figure 6: GABA regulation of transport activity is a common ALMT property as is the presence of a putative GABA-binding motif.

From: GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters

Figure 6

(a) Half-maximal effective concentration (EC50) and efficacy (Emax) of GABA regulation of rat GABAA receptors (average) or selected plant ALMT in cRNA-injected X. laevis oocytes assayed using two-electrode voltage-clamp electrophysiology (Os=rice; Hv=barley) (full data set, Supplementary Fig. 8). (b) Sequence logo of the predicted GABA-binding motif identified using MEME analysis16,18 (detailed alignment, Supplementary Fig. 9). (c) Residues corresponding to logo in proteins from a (see Supplementary Figs 10 and 11 for this sequence and residue frequencies at each position within the motif in all other identified ALMT), identical residues shaded (black), 80% similar (grey) and <60% similar (unshaded). Full-sequence identifiers are AtALMT1 (AT1G08430); TaALMT1 (DQ072260); OsALMT5 (Os04g0417000); HvALMT1 (EF424084); AtALMT13 (AT5G46600); AtALMT14 (AT5G46610); OsALMT9 (Os10g0572100); VvALMT9 (XM_002275959). All measurements were carried out at least twice with different frogs.

Back to article page