Figure 8: Muscimol reduces Arabidopsis thaliana and Vitis vinifera pollen tube elongation in vitro, and bicuculline antagonizes muscimol regulation.

(a) In vitro Arabidopsis pollen tube elongation after 3 h±treatments (n=499). Mus (20 μM muscimol and Bic (200 μM bicuculline). (b) In vitro grapevine pollen tube elongation after 6 h ± treatments, mean Δ from control mean±s.e.m. (12.5±0.16 mm) (n=157–193 per treatment). **, *** and **** indicate significant differences between genotypes at P<0.01, 0.001 and 0.0001, respectively, using a one-way analysis of variance and Tukey’s post hoc test. All error bars are ±s.e.m. (b) The experiments were replicated at least three times.