Figure 1: Investigation of the neomycin family of aminoglycosides. | Nature Communications

Figure 1: Investigation of the neomycin family of aminoglycosides.

From: Chemically related 4,5-linked aminoglycoside antibiotics drive subunit rotation in opposite directions

Figure 1

(a) 4,5-linked 2-deoxystreptamine (2-DOS) aminoglycosides in the neomycin family. (b) Overview of neomycin-binding sites in the small-subunit decoding site (h44, dark green) and in the large subunit H69 (light blue). 16S rRNA (30S) is shown in blue, 23S rRNA (50S) in grey, P-site tRNA in green and mRNA in magenta. (c) Cartoon illustrating the ribosome labelling strategy used for monitoring intersubunit rotation via single-molecule FRET, which has previously been shown to be affected by neomycin1. (d) Aminoglycoside-induced inhibition of in vitro translation. IC50 values are indicated. Experiments were performed in triplicate and the mean±s.d. is plotted.

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