Figure 3: Paromomycin effects on intersubunit rotation in wild-type and A1408G aminoglycoside-resistant ribosomes. | Nature Communications

Figure 3: Paromomycin effects on intersubunit rotation in wild-type and A1408G aminoglycoside-resistant ribosomes.

From: Chemically related 4,5-linked aminoglycoside antibiotics drive subunit rotation in opposite directions

Figure 3

(a,b) (Top panels) smFRET trajectories summed into FRET histograms reveal the population behaviours across a range of paromomycin concentrations in (a) wild-type and (b) A1408G ribosomes. (Bottom panels) Initial and final FRET values for each transition summed into two-dimensional histograms (transition density plots). Experiments were performed in triplicate on three separate days.

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