Figure 6: Neomycin and paromomycin rinse off of h44 but not H69.

Aminoglycoside real-time deliveries and rinse-outs were performed on wild-type ribosomes. For both (a) neomycin and (b) paromomycin, five population FRET histograms are shown representing the chronological order of the experiment. Left to right: (1) no drug; (2) real-time delivery of 10 μM aminoglycoside; (3) aminoglycoside equilibrium; (4) real-time rinse-out; (5) 90 s following rinse-out.